Detection of RNA-binding Proteins by In Vitro RNA Pull-down in Adipocyte Culture
Title:
Detection of RNA-binding Proteins by In Vitro RNA Pull-down in Adipocyte Culture
Author:
Bai, Qianfan
Personal Author:
Publication Information:
Cambridge, MA MyJoVE Corp 2016
Physical Description:
online resource (634 seconds)
Series:
Biology
General Note:
Title from resource description page
Abstract:
An RNA pull-down protocol is optimized here for detection of interactions between RNA-binding proteins (RBPs) and noncoding as well as coding RNAs. An RNA fragment from androgen receptor (AR) was used as an example to demonstrate how to retrieve its RBP from lystate of primary brown adipocytes.
Reading Level:
For undergraduate, graduate, and professional students
Subject Term:
Electronic Access:
https://www.jove.com/t/54207