High-throughput Functional Screening using a Homemade Dual-glow Luciferase Assay
Title:
High-throughput Functional Screening using a Homemade Dual-glow Luciferase Assay
Author:
Baker, Jessica M.
Personal Author:
Publication Information:
Cambridge, MA MyJoVE Corp 2016
Physical Description:
online resource (775 seconds)
Series:
Biology
General Note:
Title from resource description page
Abstract:
We present a rapid and inexpensive screening method for identifying transcriptional regulators using high-throughput robotic transfections and a homemade dual-glow luciferase assay. This protocol rapidly generates direct side-by-side functional data for thousands of genes and is easily modifiable to target any gene of interest.
Reading Level:
For undergraduate, graduate, and professional students
Subject Term:
Electronic Access:
https://www.jove.com/t/50282