Flow Cytometric Analysis of Extracellular Vesicles from Cell-conditioned Media
Title:
Flow Cytometric Analysis of Extracellular Vesicles from Cell-conditioned Media
Author:
Balbi, Carolina
Personal Author:
Publication Information:
Cambridge, MA MyJoVE Corp 2016
Physical Description:
online resource (512 seconds)
Series:
Biology
General Note:
Title from resource description page
Abstract:
The protocol describes a reproducible method designed for use with cell culture supernatants to detect surface epitopes on small extracellular vesicles (EV). It utilizes specific EV immunoprecipitation using beads coupled with antibodies that recognize surface antigen CD9, CD63, and CD81. The method is optimized for downstream flow cytometry analysis.
Reading Level:
For undergraduate, graduate, and professional students
Subject Term:
Electronic Access:
https://www.jove.com/t/59128