Fluorescence Activated Cell Sorting of Plant Protoplasts
Title:
Fluorescence Activated Cell Sorting of Plant Protoplasts
Author:
Bargmann, Bastiaan O. R.
Personal Author:
Publication Information:
Cambridge, MA MyJoVE Corp 2016
Physical Description:
online resource (815 seconds)
Series:
Biology
General Note:
Title from resource description page
Abstract:
A method for isolating specific cell types from plant material is demonstrated. This technique employs transgenic marker lines expressing fluorescent proteins in particular cell types, cellular dissociation and Fluorescence Activated Cell Sorting. Additionally, a growth setup is established here that facilitates treatment of Arabidopsis thaliana seedlings prior to cell sorting.
Reading Level:
For undergraduate, graduate, and professional students
Subject Term:
Electronic Access:
https://www.jove.com/t/1673