Application of Laser Microdissection to Uncover Regional Transcriptomics in Human Kidney Tissue
Title:
Application of Laser Microdissection to Uncover Regional Transcriptomics in Human Kidney Tissue
Author:
Barwinska, Daria
Personal Author:
Publication Information:
Cambridge, MA MyJoVE Corp 2016
Physical Description:
online resource (346 seconds)
Series:
Biology
General Note:
Title from resource description page
Abstract:
We describe a protocol for laser microdissection of sub-segments of the human kidney, including the glomerulus, proximal tubule, thick ascending limb, collecting duct and interstitium. The RNA is then isolated from the obtained compartments and RNA sequencing is carried out to determine changes in the transcriptomic signature within each sub-segment.
Reading Level:
For undergraduate, graduate, and professional students
Subject Term:
Electronic Access:
https://www.jove.com/t/61371