CONTENTS -- Preface -- Introduction to the 14th Symposium -- PART 1. LUCIFERASES -- Refolding of the recombinant luciferases of Metridia longa Borisova VV, Frank LA, Markova SV, Golz S and Vysotski ES -- INTRODUCTION -- MATERIALS AND METHODS -- RESULTS AND DISCUSSION -- ACKNOWLEDGEMENTS -- REFERENCES -- The main fuction of HIS175, TRP179, and TYR190 residues of the obelin binding site is to stabilize the hydroperoxycoelenterazine intermediate Eremeeva EV, Markova SV, Frank LA and Vysotski ES -- INTRODUCTION -- MATERIALS AND METHODS -- RESULTS AND DISCUSSION -- CONCLUSIONS -- ACKNOWLEDGEMENTS -- REFERENCES -- Mechanistic study on the Cypridina (Vargula) bioluminescence reaction Hirano T, Takahashi Y, Kondo H, Ikeda H, Maki S and Niwa H -- INTRODUCTION -- METHODS -- RESULTS AND DISCUSSION -- REFERENCES -- Stabilization of Luciola mingrelica firefly luciferase by mutation of non-conservative cysteine residues Lomakina GY, Legotsky SA, Tishkov VI and Ugarova NN -- INTRODUCTION -- METHODS -- RESULTS AND DISCUSSION -- ACKNOWLEDGEMENTS -- REFERENCES -- Firefly luciferase arose from fatty acyl-CoA synthetase by gene duplication and gene recruitment Oba Y and Inouye S -- INTRODUCTION -- MATERIALS AND METHODS -- RESULTS -- DISCUSSION -- REFERENCES -- Bioluminescence in click beetles (family Elateridae): Molecular phylogenetic analysis, inferred from 28S ribosomal DNA Sagegami-Oba R, Oba Y and Ohira H -- INTRODUCTION -- MATERIALS AND METHODS -- RESULTS AND DISCUSSION -- REFERENCES -- Effect of microenvironmental changes on kinetic parameters of steady-state enzyme-induced bacterial bioluminescent reaction Sukovataya IE, Tyulkova NA and Kaykova EV -- INTRODUCTION -- METHODS -- RESULTS -- ACKNOWLEDGEMENTS -- REFERENCES.

Thermal stability and absorption spectra of bacterial luciferases from Photobacterium leiognathi in water-organic solvents Sukovataya IE, Tyulkova NA and Kaykova EV -- INTRODUCTION -- METHODS -- RESULTS -- ACKNOWLEDGEMENTS -- REFERENCES -- Characterization of a fish symbiont of Photobacterium phosphoreum with altered spectral properties Whitaker S, Herring PJ, Campbell AK, Hill PJ and Rees CED -- INTRODUCTION -- MATERIALS AND METHODS -- RESULTS -- REFERENCES -- PART 2. INSTRUMENTATION -- Luminometers for industrial applications of rapid microbiology Berthold F, Haher K, Schreibers M and Tarkkanen V -- HISTORICAL PERSPECTIVE -- MICROBIAL CONTAMINATION SCREENING IN THE FOOD INDUSTRY -- PROBLEMS IN INDUSTRIAL HIGH THROUGHPUT INSTRUMENTS -- LATEST DEVELOPMENTS -- ACKNOWLEDGEMENTS -- REFERENCES -- Application of a luminescence microscope with novel optical system for detection of the gene expression pattern of individual cells Hatta-Ohashi Y, Hayasaka N, Takahasi T, Shigeyoshi Y and Suzuki H -- INTRODUCTION -- MATERIALS AND METHODS -- RESULTS AND DISCUSSION -- REFERENCES -- Novel tools for quantification of luminescence Hickey PC -- INTRODUCTION -- MATERIALS AND METHODS -- RESULTS AND DISCUSSION -- CONCLUSIONS -- ACKNOWLEDGEMENTS -- REFERENCES -- Luminescence microscope for reporter assay of single live cells Suzuki H, Dosaka S, Ohashi-Hatta Y and Sugiyama T -- INTRODUCTION -- METHODS -- RESULTS AND DISCUSSION -- REFERENCES -- PART 3. ASSAYS -- Elevation of choline concentration in cardiac troponin-I positive human plasma Adamczyk M, Brashear RJ and Mattingly PG -- INTRODUCTION -- MATERIALS AND METHODS -- RESULTS AND DISCUSSION -- CONCLUSIONS -- REFERENCES -- High throughput detection of hydrogen peroxide: Validation of homogeneous chemiluminescent assays for choline in human plasma and whole blood Adamczyk M, Brashear RJ, Mattingly PG and Tsatsos PH.

INTRODUCTION -- MATERIALS AND METHODS -- RESULTS AND DISCUSSION -- CONCLUSIONS -- REFERENCES -- Development of bioluminescent pyrophosphate assay using pyruvate phosphate dikinase and its application to SNPs analysis Arakawa H, Munakata E, Obinata R, Maeda M, Suzuki S, Kamahori M and Kambara H -- INTRODUCTION -- MATERIALS AND METHODS -- RESULTS AND DISCUSSION -- REFERENCES -- Bioluminescent method for detection of proteinase activity in UHT milk Brovko LY, Hajdok C, Tapp C, Ramsay C, Driscoll M and Griflths MW -- INTRODUCTION -- MATERIALS AND METHODS -- RESULTS AND DISCUSSION -- REFERENCES -- Detection and control of Salmonella typhimurium growth using specific bacteriophage Brovko LY, Romanova NA, Allain B, Mandeville R and Grifiths MW -- INTRODUCTION -- MATERIALS AND METHODS -- RESULTS AND DISCUSSION -- REFERENCES -- Improved sensitivity method for rapid hygiene monitoring using ATP bioluminescence Driscoll M, Ramsay C and Watkin J -- INTRODUCTION -- MATERIALS AND METHODS -- RESULTS AND DISCUSSION -- REFERENCES -- Determination of antioxidant capacity and reactivity using hemin-catalyzed luminol chemiluminescence Eckert CR, Esteves CH, Bastos EL, Romof P and Baader WJ -- INTRODUCTION -- MATERIAL AND METHODS -- RESULTS AND DISCUSSION -- ACKNOWLEDGEMENTS -- REFERENCES -- Applications of adenylate kinase detection using bioluminescence Foote N, Green M and Gregory S -- INTRODUCTION -- METHODS -- RESULTS -- CONCLUSIONS -- REFERENCES -- Standardization of bioluminescent raw milk quality assay using Lumtek test-systems Frundzhyan VG, Ugarova NN, Makarushin AA and Gavrilov GB -- INTRODUCTION -- MATERIALS AND METHODS -- RESULTS -- REFERENCES -- BART - Bioluminescent alternative to real-time PCR Gandelman 0, Church VL, Moore CA, Came C, Jalal H, Murray JAH and Tisi LC -- INTRODUCTION -- METHODS -- RESULTS AND DISCUSSION -- REFERENCES.

Advancing the development of dual-luciferase assays Hawkins EM, Gawin D, Paguio A, Stecha P, Swanson B, Fan F and Wood KV -- INTRODUCTION -- MODEL FOR ANALYSIS OF GPROTEIN COUPLED RECEPTORS -- DUAL PLASMID STRATEGY FOR STABLE CELL LINES -- GENE CONSTRUCTS -- SYSTEM TESTING -- CONCLUSION -- Simultaneous bioluminescent immunoassay using aequorin labeled Fab fragment and biotinylated firefly luciferase Ito K, Nishimura W, Maeda M, Gomi K, Inouye S and Arakawa H -- INTRODUCTION -- MATERIALS AND METHODS -- RESULTS AND DISSCUSSION -- ACKNOWLEDGEMENTS -- REFFERENCES -- Designing enhanced thermostable luciferase for proteolytic assay Jathoul AP, Gandelman OA, Law GHE, Murray JAH and Tisi LC -- INTRODUCTION -- MATERIALS AND METHODS -- RESULTS AND DISCUSSION -- CONCLUSIONS -- REFERENCES -- Biosensors for the non-invasive evaluation of bacterial quorum sensing in GI disorders Kumari A, Pasini P, Deo SK, Flomenhoft D, Shashidhar H and Daunert S -- INTRODUCTION -- EXPERIMENTAL -- RESULTS AND DISCUSSION -- ACKNOWLEDGMENTS -- REFERENCES -- Improved Ca2+-activated photoproteins for HTS applications Mastroianni N, Flak T and Corazza S -- INTRODUCTION -- METHODS -- RESULTS AND DISCUSSION -- CONCLUSIONS -- REFERENCES -- Development of a multiplexed bioluminescent cell-based assay with the luc gene from Luciola italica for high throughput screening of cholesterol-lowering drugs Michelini E, Southworth TL, Ablamski D, Branchini BR and Roda A -- INTRODUCTION -- MATERIALS AND METHODS -- CONCLUSIONS -- REFERENCES -- Novel BET-based biosensors with a new bioluminescent donor, Gaussia luciferase, for estrogen receptor ligands Michelini E, Magliulo M, Baraldini M and Roda A -- INTRODUCTION -- MATERIALS AND METHODS -- RESULTS AND DISCUSSION -- CONCLUSIONS -- REFERENCES.

The use of lux genes for monitoring uptake of Pseudomonas aeruginosa by respiratory epithelial cells Robinson GM, Jackson SK, Salisbury VC and Nelson SM -- INTRODUCTION -- METHOD -- RESULTS -- DISCUSSION -- REFERENCES -- Bioluminescence characteristics of an Obelin mutant in varying solvent conditions Rowe L, Rothert A, Deo SK, Ensor M and Daunert S -- INTRODUCTION -- MATERIALS AND METHODS -- RESULTS AND DISCUSSION -- ACKNOWLEDGEMENTS -- REFERENCES -- A simple chemiluminescence flow injection analysis for the determination of sulphide in aqueous samples Satienperakul S, Liawruangrath S and Cardwell TJ -- INTRODUCTION -- MATERIALS AND METHODS -- RESULTS AND DISCUSSION -- CONCLUSIONS -- REFERENCES -- Using recombinant antigens for the detection of antibodies to Trypanosomas cruzi on a fully automated chemiluminescence analyzer Shah D, Chang C-D, Cheng K, Jiang L, Salbilla V, Haller A and Schochetman G -- INTRODUCTION -- MATERIALS AND METHODS -- RESULTS AND DISCUSSION -- ACKNOWLEDGEMENTS -- REFERENCES -- Quantitative bioluminescent RNA assay Sun Y, Jacobson KB and Golovlev V -- INTRODUCTION -- RESULTS -- DISCUSSION -- ACKNOWLEDGEMENTS -- REFERENCES -- A novel multi-channel chemiluminescence-ioassay to detect HIV antibodies as a more specific supplemental test Sun X and Yang X -- INTRODUCTION -- MATERIALS AND METHODS -- RESULTS AND DISCUSSION -- ACKNOWLEDGEMENTS -- REFERENCES -- Time-resolved measurement of firefly bioluminescence using photolysis of caged-ATP Tanaka M, Igarashi R and Wada N -- INTRODUCTION -- EXPERIMENTAL -- RESULTS AND DISCUSSION -- ACKNOWLEDGEMENTS -- REFERENCES -- Red-chemiluminescent probes for detecting superoxide anions Teranishi K -- INTRODUCTION -- MATERIALS AND METHODS -- RESULTS -- REFERENCES -- Aequorin bioluminescence dissected by random mutagenesis Tricoire L, Tsuzuki K and Lambolez B -- INTRODUCTION.

MATERIALS AND METHODS.