Surface Passivation for Single-molecule Protein Studies
Title:
Surface Passivation for Single-molecule Protein Studies
Author:
Chandradoss, Stanley D.
Personal Author:
Publication Information:
Cambridge, MA MyJoVE Corp 2016
Physical Description:
online resource (635 seconds)
Series:
Chemistry
General Note:
Title from resource description page
Abstract:
We describe a method for passivating a glass surface using polyethylene glycol (PEG). This protocol covers surface cleaning, surface functionalization, and PEG coating. We introduce a new strategy for treating the surface with PEG molecules over two rounds, which yields superior quality of passivation compared to existing methods.
Reading Level:
For undergraduate, graduate, and professional students
Subject Term:
Electronic Access:
https://www.jove.com/t/50549