Generation of Naïve Blastoderm Explants from Zebrafish Embryos
Title:
Generation of Naïve Blastoderm Explants from Zebrafish Embryos
Author:
Alaniz Emig, Alyssa
Personal Author:
Publication Information:
Cambridge, MA MyJoVE Corp 2016
Physical Description:
online resource (441 seconds)
Series:
Developmental Biology
General Note:
Title from resource description page
Abstract:
Zebrafish blastoderm explants are generated by isolating embryonic cells from endogenous signaling centers within the early embryo, producing relatively naïve cell clusters easily manipulated and cultured ex vivo. This article provides instructions for making such explants and demonstrates their utility by interrogating roles for Nodal signaling during gastrulation.
Reading Level:
For undergraduate, graduate, and professional students
Subject Term:
Electronic Access:
https://www.jove.com/t/62797