Incorporating Pericytes into an Endothelial Cell Bead Sprouting Assay
Title:
Incorporating Pericytes into an Endothelial Cell Bead Sprouting Assay
Author:
Azam, Salma H.
Personal Author:
Publication Information:
Cambridge, MA MyJoVE Corp 2016
Physical Description:
online resource (381 seconds)
Series:
Developmental Biology
General Note:
Title from resource description page
Abstract:
This protocol presents a novel in vitro bead assay that more appropriately models the process of in vivo sprouting angiogenesis by incorporating pericytes. This modification enables the bead assay to more faithfully recapitulate the heterotypic cellular interactions between endothelial cells and mural cells that are critical for angiogenesis.
Reading Level:
For undergraduate, graduate, and professional students
Subject Term:
Electronic Access:
https://www.jove.com/t/57309