A Co-Culture Method to Study Neurite Outgrowth in Response to Dental Pulp Paracrine Signals
Title:
A Co-Culture Method to Study Neurite Outgrowth in Response to Dental Pulp Paracrine Signals
Author:
Barkley, Courtney
Personal Author:
Publication Information:
Cambridge, MA MyJoVE Corp 2016
Physical Description:
online resource (486 seconds)
Series:
Developmental Biology
General Note:
Title from resource description page
Abstract:
We describe the isolation, dispersion and plating of dental pulp (DP) primary cells with trigeminal (TG) neurons cultured atop overlying transwell filters. Cellular responses of DP cells can be analyzed with immunofluorescence or RNA/protein analysis. Immunofluorescence of neuronal markers with confocal microscopy permits the analysis of neurite outgrowth responses.
Reading Level:
For undergraduate, graduate, and professional students
Subject Term:
Electronic Access:
https://www.jove.com/t/60809