Immunostaining to Visualize Murine Enteric Nervous System Development
Title:
Immunostaining to Visualize Murine Enteric Nervous System Development
Author:
Barlow-Anacker, Amanda J.
Personal Author:
Publication Information:
Cambridge, MA MyJoVE Corp 2016
Physical Description:
online resource (474 seconds)
Series:
Developmental Biology
General Note:
Title from resource description page
Abstract:
The enteric nervous system is formed by neural crest cells that proliferate, migrate and colonize the gut. Neural crest cells differentiate into neurons with markers specific for their neurotransmitter phenotype. This protocol describes a technique for dissecting, fixing and immunostaining of the murine embryonic gastrointestinal tract to visualize enteric nervous system neurotransmitter expression.
Reading Level:
For undergraduate, graduate, and professional students
Subject Term:
Electronic Access:
https://www.jove.com/t/52716