Establishment of Genome-edited Human Pluripotent Stem Cell Lines: From Targeting to Isolation
Title:
Establishment of Genome-edited Human Pluripotent Stem Cell Lines: From Targeting to Isolation
Author:
Blair, John D.
Personal Author:
Publication Information:
Cambridge, MA MyJoVE Corp 2016
Physical Description:
online resource (591 seconds)
Series:
Developmental Biology
General Note:
Title from resource description page
Abstract:
Genome editing of human pluripotent stem cells (hPSCs) can be done quickly and efficiently. Presented here is a robust experimental procedure to genetically engineer hPSCs as exemplified by editing the AAVS1 safe harbor locus to express EGFP and introduce antibiotic resistance.
Reading Level:
For undergraduate, graduate, and professional students
Subject Term:
Electronic Access:
https://www.jove.com/t/53583