Tracing Gene Expression Through Detection of β-galactosidase Activity in Whole Mouse Embryos
Title:
Tracing Gene Expression Through Detection of β-galactosidase Activity in Whole Mouse Embryos
Author:
Blanco, María José
Personal Author:
Publication Information:
Cambridge, MA MyJoVE Corp 2016
Physical Description:
online resource (522 seconds)
Series:
Developmental Biology
General Note:
Title from resource description page
Abstract:
Here we describe the standard protocol for the detection of β-galactosidase activity in early whole mouse embryos and the method for paraffin sectioning and counterstaining. This is an easy and quick procedure to monitor gene expression during development that can also be applied to tissue sections, organs or cultured cells.
Reading Level:
For undergraduate, graduate, and professional students
Subject Term:
Electronic Access:
https://www.jove.com/t/57785