Isolation and Characterization of Satellite Cells from Rat Head Branchiomeric Muscles
Title:
Isolation and Characterization of Satellite Cells from Rat Head Branchiomeric Muscles
Author:
Carvajal Monroy, Paola L.
Personal Author:
Publication Information:
Cambridge, MA MyJoVE Corp 2016
Physical Description:
online resource (457 seconds)
Series:
Developmental Biology
General Note:
Title from resource description page
Abstract:
This protocol describes the isolation of satellite cells from branchiomeric head muscles of a 9 week-old rat. The muscles originate from different branchial arches. Subsequently, the satellite cells are cultured on a spot coating of millimeter size to study their differentiation. This approach avoids the expansion and passaging of satellite cells.
Reading Level:
For undergraduate, graduate, and professional students
Subject Term:
Electronic Access:
https://www.jove.com/t/52802