Hepatocyte-specific Ablation in Zebrafish to Study Biliary-driven Liver Regeneration
Title:
Hepatocyte-specific Ablation in Zebrafish to Study Biliary-driven Liver Regeneration
Author:
Choi, Tae-Young
Personal Author:
Publication Information:
Cambridge, MA MyJoVE Corp 2016
Physical Description:
online resource (494 seconds)
Series:
Developmental Biology
General Note:
Title from resource description page
Abstract:
To help assess the molecular mechanisms underlying zebrafish biliary-driven liver regeneration, we established a liver injury model in which the nitroreductase-expressing hepatocytes are genetically ablated upon metronidazole treatment. In this protocol, we describe how to adeptly manipulate, monitor and analyze hepatocyte ablation and biliary-driven liver regeneration.
Reading Level:
For undergraduate, graduate, and professional students
Subject Term:
Electronic Access:
https://www.jove.com/t/52785