Scalable 96-well Plate Based iPSC Culture and Production Using a Robotic Liquid Handling System
Title:
Scalable 96-well Plate Based iPSC Culture and Production Using a Robotic Liquid Handling System
Author:
Conway, Michael K.
Personal Author:
Publication Information:
Cambridge, MA MyJoVE Corp 2016
Physical Description:
online resource (480 seconds)
Series:
Developmental Biology
General Note:
Title from resource description page
Abstract:
The protocols described allow laboratories to perform scalable, adherent stem cell culture in high throughput with minimal labor, experience and equipment investment cost using a programmable liquid handling robot and 96-well plates. iPSCs passaged more than 20 times on this system maintained pluripotency, normal karyotypes and differentiated into cardiomyocytes.
Reading Level:
For undergraduate, graduate, and professional students
Subject Term:
Electronic Access:
https://www.jove.com/t/52755