Small-scale Propagation of Human iPSCs in Serum-free Conditions for Routine Immunocytochemical Characterization
Title:
Small-scale Propagation of Human iPSCs in Serum-free Conditions for Routine Immunocytochemical Characterization
Author:
Corenblum, Mandi J.
Personal Author:
Publication Information:
Cambridge, MA MyJoVE Corp 2016
Physical Description:
online resource (596 seconds)
Series:
Developmental Biology
General Note:
Title from resource description page
Abstract:
Regular characterization of induced pluripotent stem cells (iPSCs), to ascertain maintenance of their pluripotent state, is an important step before these cells are used for other applications. Here we describe a method for the small-scale propagation of human iPSCs specifically designed to enable their easy and routine characterization via immunocytochemistry.
Reading Level:
For undergraduate, graduate, and professional students
Subject Term:
Electronic Access:
https://www.jove.com/t/55260