Generation of Genetically Modified Mice through the Microinjection of Oocytes
Title:
Generation of Genetically Modified Mice through the Microinjection of Oocytes
Author:
Delerue, Fabien
Personal Author:
Publication Information:
Cambridge, MA MyJoVE Corp 2016
Physical Description:
online resource (619 seconds)
Series:
Developmental Biology
General Note:
Title from resource description page
Abstract:
The microinjection of mouse oocytes is commonly used for both classic transgenesis (i.e., the random integration of transgenes) and CRISPR-mediated gene targeting. This protocol reviews the latest developments in microinjection, with a particular emphasis on quality control and genotyping strategies.
Reading Level:
For undergraduate, graduate, and professional students
Subject Term:
Electronic Access:
https://www.jove.com/t/55765