Isolating and Analyzing Cells of the Pancreas Mesenchyme by Flow Cytometry
Title:
Isolating and Analyzing Cells of the Pancreas Mesenchyme by Flow Cytometry
Author:
Epshtein, Alona
Personal Author:
Publication Information:
Cambridge, MA MyJoVE Corp 2016
Physical Description:
online resource (338 seconds)
Series:
Developmental Biology
General Note:
Title from resource description page
Abstract:
Here, we describe a method for the isolation of cells in the pancreas microenvironment from embryonic, neonatal and adult mouse tissue, focusing on the isolation of mesenchymal cells. This method allows profiling of cell gene expression and protein secretion in order to elucidate the extrinsic signals that regulate pancreas development, function, and tumorigenesis.
Reading Level:
For undergraduate, graduate, and professional students
Subject Term:
Electronic Access:
https://www.jove.com/t/55344