Microinjection for Transgenesis and Genome Editing in Threespine Sticklebacks
Title:
Microinjection for Transgenesis and Genome Editing in Threespine Sticklebacks
Author:
Erickson, Priscilla A.
Personal Author:
Publication Information:
Cambridge, MA MyJoVE Corp 2016
Physical Description:
online resource (531 seconds)
Series:
Developmental Biology
General Note:
Title from resource description page
Abstract:
Transgenic manipulations and genome editing are critical for functionally testing the roles of genes and cis-regulatory elements. Here a detailed microinjection protocol for the generation of genomic modifications (including Tol2-mediated fluorescent reporter transgene constructs, TALENs, and CRISPRs) is presented for the emergent model fish, the threespine stickleback.
Reading Level:
For undergraduate, graduate, and professional students
Subject Term:
Electronic Access:
https://www.jove.com/t/54055