Isolation of Murine Embryonic Hemogenic Endothelial Cells
Title:
Isolation of Murine Embryonic Hemogenic Endothelial Cells
Author:
Fang, Jennifer S.
Personal Author:
Publication Information:
Cambridge, MA MyJoVE Corp 2016
Physical Description:
online resource (536 seconds)
Series:
Developmental Biology
General Note:
Title from resource description page
Abstract:
Hematopoietic stem and progenitor cells (HSPC) derive from specialized (hemogenic) endothelial cells during development, yet little is known about the process by which some endothelial cells specify to become blood forming. We demonstrate a flow-cytometry based method allowing simultaneous isolation of hemogenic endothelial cells and HSPC from murine embryonic tissues.
Reading Level:
For undergraduate, graduate, and professional students
Subject Term:
Electronic Access:
https://www.jove.com/t/54150