Analysis of Chromosome Segregation, Histone Acetylation, and Spindle Morphology in Horse Oocytes
Title:
Analysis of Chromosome Segregation, Histone Acetylation, and Spindle Morphology in Horse Oocytes
Author:
Franciosi, Federica
Personal Author:
Publication Information:
Cambridge, MA MyJoVE Corp 2016
Physical Description:
online resource (731 seconds)
Series:
Developmental Biology
General Note:
Title from resource description page
Abstract:
This manuscript describes an experimental approach to morphologically and biochemically characterize horse oocytes. Specifically, the present work illustrates how to collect immature and mature horse oocytes by ultrasound-guided ovum pick-up (OPU) and how to investigate chromosome segregation, spindle morphology, global histone acetylation, and mRNA expression.
Reading Level:
For undergraduate, graduate, and professional students
Subject Term:
Electronic Access:
https://www.jove.com/t/55242