Differentiation and Characterization of Neural Progenitors and Neurons from Mouse Embryonic Stem Cells
Title:
Differentiation and Characterization of Neural Progenitors and Neurons from Mouse Embryonic Stem Cells
Author:
Hanafiah, Aflah
Personal Author:
Publication Information:
Cambridge, MA MyJoVE Corp 2016
Physical Description:
online resource (527 seconds)
Series:
Developmental Biology
General Note:
Title from resource description page
Abstract:
We describe the procedure for the in vitro differentiation of mouse embryonic stem cells into neuronal cells using the hanging drop method. Furthermore, we perform a comprehensive phenotypic analysis through RT-qPCR, immunofluorescence, RNA-seq, and flow cytometry.
Reading Level:
For undergraduate, graduate, and professional students
Subject Term:
Electronic Access:
https://www.jove.com/t/61446