Culture of Embryonic Mouse Cochlear Explants and Gene Transfer by Electroporation
Title:
Culture of Embryonic Mouse Cochlear Explants and Gene Transfer by Electroporation
Author:
Haque, Khujista D.
Personal Author:
Publication Information:
Cambridge, MA MyJoVE Corp 2016
Physical Description:
online resource (543 seconds)
Series:
Developmental Biology
General Note:
Title from resource description page
Abstract:
We present a method that describes isolation and culture of cochlear explants from embryonic mouse inner ear. We also demonstrate a method for gene transfer into cochlear explants via square-wave electroporation. The in vitro explant culture coupled with gene transfer technique enables researchers to study the effects of altering gene expression during development.
Reading Level:
For undergraduate, graduate, and professional students
Subject Term:
Electronic Access:
https://www.jove.com/t/52260