Induction and Validation of Cellular Senescence in Primary Human Cells
Title:
Induction and Validation of Cellular Senescence in Primary Human Cells
Author:
Hernandez-Segura, Alejandra
Personal Author:
Publication Information:
Cambridge, MA MyJoVE Corp 2016
Physical Description:
online resource (498 seconds)
Series:
Developmental Biology
General Note:
Title from resource description page
Abstract:
Here, we discuss a series of protocols for induction and validation of cellular senescence in cultured cells. We focus on different senescence-inducing stimuli and describe the quantification of common senescence-associated markers. We provide technical details using fibroblasts as a model, but the protocols can be adapted to various cellular models.
Reading Level:
For undergraduate, graduate, and professional students
Subject Term:
Electronic Access:
https://www.jove.com/t/57782