Cover -- Title -- Copyright -- Contents -- Introduction -- Chapter 1: Cloning Defined and Major Developments in the History of Cloning -- Cloning in Nature -- Natural Cloning Processes in Humans -- Natural Cloning Processes in Plants -- Regeneration -- Artificial Cloning -- Early Cloning Experiments -- Sir John Bertrand Gurdon -- The Development of Recombinant DNA Technology -- The First Cloned Mammals -- Cloning Experiments with Human Embryos -- Sir Ian Wilmut -- Education and Cryopreservation Research -- Genetic Engineering and Cloning Research -- Later Career -- Developments After Dolly -- A New Kind of Stem Cell -- Shinya Yamanaka -- Chapter 2: Gene Cloning, DNA Analysis, and Gene Sequencing -- Gene Cloning -- Creating the Clone -- Isolating the Clone -- DNA Sequencing -- Uses of DNA Sequencing -- Methods of DNA Sequencing -- Paul Berg -- In Vitro Mutagenesis -- Gene Cloning and Genetically Modified Organisms -- Reverse Genetics -- Diagnostics -- Protein Manufacture -- Genomics -- Sequencing and Analysis of Genomes -- Genomics Applications -- Chapter 3: An Overview of Animal and Plant Reproduction Processes -- Reproductive Systems of Invertebrates -- Reproductive Systems of Vertebrates -- Parthenogenesis -- The Testes -- The Ovaries -- Ovulation -- Reproduction in Plants -- Early Development and Cell Differentiation -- Chapter 4: Reproductive Cloning and Genetic Engineering -- Dolly, the First Clone of an Adult Mammal -- Somatic Cell Nuclear Transfer -- Frozen Ark -- Genetically Modified Organisms -- GMOs in Agriculture -- GMOs in Medicine and Research -- Pharming -- Role of GMOs in Environmental Management -- De-Extinction -- The Commercialization of GMOs -- Chapter 5: Therapeutic Cloning -- Stem Cells from Human Embryos -- Regenerative Medicine and Stem Cells -- Hwang Woo-Suk -- Cell and Bioartificial Tissue Transplantation.

Regeneration Using Stem Cells -- James Thomson -- Tissue Scaffolds and Soluble Repair Factors -- Replacing Diseased Tissue -- Therapeutic Cloning and Gene Therapy -- Chapter 6: Ethical and Moral Issues -- Reproductive Cloning of Humans -- The Uses and Ethics of Cloning, by Ian Wilmut (1997) -- The Use of Human Embryos for Stem Cell Research -- Ethical and Social Issues: GMOs, De-Extinction, and Gene Therapy -- Sociopolitical Relevance of GMOs -- Ethical Considerations of De-Extinction -- Concerns Related to Therapeutic Cloning for Gene Therapy -- Conclusion -- Glossary -- Bibliography -- Index -- Back Cover.