Culture of Small Colony Variant of Pseudomonas aeruginosa and Quantitation of its Alginate
Title:
Culture of Small Colony Variant of Pseudomonas aeruginosa and Quantitation of its Alginate
Author:
Al Ahmar, Roy
Personal Author:
Publication Information:
Cambridge, MA MyJoVE Corp 2016
Physical Description:
online resource (546 seconds)
Series:
Immunology and Infection
General Note:
Title from resource description page
Abstract:
Here, we describe a growth condition to culture the small colony variant of Pseudomonas aeruginosa. We also describe two separate methods for the detection and quantitation of the exopolysaccharide alginate produced by P. aeruginosa using a traditional uronic acid carbazole assay and an alginate-specific monoclonal antibody (mAb) based ELISA.
Reading Level:
For undergraduate, graduate, and professional students
Subject Term:
Electronic Access:
https://www.jove.com/t/60466