Flow Virometry to Analyze Antigenic Spectra of Virions and Extracellular Vesicles
Title:
Flow Virometry to Analyze Antigenic Spectra of Virions and Extracellular Vesicles
Author:
Arakelyan, Anush
Personal Author:
Publication Information:
Cambridge, MA MyJoVE Corp 2016
Physical Description:
online resource (388 seconds)
Series:
Immunology and Infection
General Note:
Title from resource description page
Abstract:
Viruses or extracellular vesicles were immunocaptured with 15 nm magnetic nanoparticles coupled to antibodies recognizing surface antigens. The captured virions or vesicles were labeled with fluorescent antibodies against other surface antigens. The resultant complexes were separated in high magnetic field and analyzed with conventional flow cytometers triggered on fluorescence.
Reading Level:
For undergraduate, graduate, and professional students
Subject Term:
Electronic Access:
https://www.jove.com/t/55020