Isolation of Leukocytes from the Murine Tissues at the Maternal-Fetal Interface
Title:
Isolation of Leukocytes from the Murine Tissues at the Maternal-Fetal Interface
Author:
Arenas-Hernandez, Marcia
Personal Author:
Publication Information:
Cambridge, MA MyJoVE Corp 2016
Physical Description:
online resource (471 seconds)
Series:
Immunology and Infection
General Note:
Title from resource description page
Abstract:
Described herein is a protocol to isolate and analyze the infiltrating leukocytes of tissues at the maternal-fetal interface (uterus, decidua, and placenta) of mice. This protocol maintains the integrity of most cell surface markers and yields enough viable cells for downstream applications including flow cytometry analysis.
Reading Level:
For undergraduate, graduate, and professional students
Subject Term:
Electronic Access:
https://www.jove.com/t/52866