A High-throughput Assay to Assess and Quantify Neutrophil Extracellular Trap Formation
Title:
A High-throughput Assay to Assess and Quantify Neutrophil Extracellular Trap Formation
Author:
Arends, Eline J.
Personal Author:
Publication Information:
Cambridge, MA MyJoVE Corp 2016
Physical Description:
online resource (599 seconds)
Series:
Immunology and Infection
General Note:
Title from resource description page
Abstract:
This protocol describes a highly sensitive and high throughput neutrophil extracellular trap (NET) assay for the semi-automated quantification of ex vivo NET formation by immunofluorescence three-dimensional confocal microscopy. This protocol can be used to evaluate NET formation and degradation after different stimuli and can be used to study potential NET-targeted therapies.
Reading Level:
For undergraduate, graduate, and professional students
Subject Term:
Electronic Access:
https://www.jove.com/t/59150