Isolation and Identification of Extravascular Immune Cells of the Heart
Title:
Isolation and Identification of Extravascular Immune Cells of the Heart
Author:
Aronoff, Laura
Personal Author:
Publication Information:
Cambridge, MA MyJoVE Corp 2016
Physical Description:
online resource (504 seconds)
Series:
Immunology and Infection
General Note:
Title from resource description page
Abstract:
This protocol presents a simple and efficient method to isolate, identify and quantify immune cells residing in the myocardium of mice during steady state or inflammation. The protocol combines enzymatic and mechanical digestion for the generation of a single cell suspension that can be further analyzed by flow cytometry.
Reading Level:
For undergraduate, graduate, and professional students
Subject Term:
Electronic Access:
https://www.jove.com/t/58114