Investigating Mast Cell Secretory Granules; from Biosynthesis to Exocytosis
Title:
Investigating Mast Cell Secretory Granules; from Biosynthesis to Exocytosis
Author:
Azouz, Nurit P.
Personal Author:
Publication Information:
Cambridge, MA MyJoVE Corp 2016
Physical Description:
online resource (961 seconds)
Series:
Immunology and Infection
General Note:
Title from resource description page
Abstract:
The goal of the present protocol was to develop a method that will allow functional genomic analyses of mast cell secretion. The protocol is based on quantitative assessment of the release of a fluorescent reporter gene cotrasfected with the gene of interest and real time analyses of the secretory granule's morphology.
Reading Level:
For undergraduate, graduate, and professional students
Subject Term:
Electronic Access:
https://www.jove.com/t/52505