Isolation of Macrophage Subsets and Stromal Cells from Human and Mouse Myocardial Specimens
Title:
Isolation of Macrophage Subsets and Stromal Cells from Human and Mouse Myocardial Specimens
Author:
Bajpai, Geetika
Personal Author:
Publication Information:
Cambridge, MA MyJoVE Corp 2016
Physical Description:
online resource (445 seconds)
Series:
Immunology and Infection
General Note:
Title from resource description page
Abstract:
Presented here is a protocol to isolate various subsets of macrophages and other non-immune cells from human and mouse myocardium by preparing a single cell suspension through enzymatic digestion. Gating schemes for flow cytometry based identification and characterization of isolated macrophages are also presented.
Reading Level:
For undergraduate, graduate, and professional students
Subject Term:
Electronic Access:
https://www.jove.com/t/60015