Measurement of In Vitro Integration Activity of HIV-1 Preintegration Complexes
Title:
Measurement of In Vitro Integration Activity of HIV-1 Preintegration Complexes
Author:
Balasubramaniam, Muthukumar
Personal Author:
Publication Information:
Cambridge, MA MyJoVE Corp 2016
Physical Description:
online resource (634 seconds)
Series:
Immunology and Infection
General Note:
Title from resource description page
Abstract:
This report describes an in vitro assay for measuring the human immunodeficiency virus type 1 (HIV-1) preintegration complex (PIC)-associated integration activity in the cytoplasmic extracts of acutely infected cells. The integration of PIC-associated HIV-1 DNA into heterologous target DNA is quantified by using a nested real-time polymerase chain reaction strategy.
Reading Level:
For undergraduate, graduate, and professional students
Subject Term:
Electronic Access:
https://www.jove.com/t/54581