Label-Free Quantitative Proteomics Workflow for Discovery-Driven Host-Pathogen Interactions
Title:
Label-Free Quantitative Proteomics Workflow for Discovery-Driven Host-Pathogen Interactions
Author:
Ball, Brianna
Personal Author:
Publication Information:
Cambridge, MA MyJoVE Corp 2016
Physical Description:
online resource (337 seconds)
Series:
Immunology and Infection
General Note:
Title from resource description page
Abstract:
Here, we present a protocol to profile the interplay between host and pathogen during infection by mass spectrometry-based proteomics. This protocol uses label-free quantification to measure changes in protein abundance of both host (e.g., macrophages) and pathogen (e.g., Cryptococcus neoformans) in a single experiment.
Reading Level:
For undergraduate, graduate, and professional students
Subject Term:
Electronic Access:
https://www.jove.com/t/61881