Propagation of Homalodisca coagulata virus-01 via Homalodisca vitripennis Cell Culture
Title:
Propagation of Homalodisca coagulata virus-01 via Homalodisca vitripennis Cell Culture
Author:
Biesbrock, Anna M.
Personal Author:
Publication Information:
Cambridge, MA MyJoVE Corp 2016
Physical Description:
online resource (772 seconds)
Series:
Immunology and Infection
General Note:
Title from resource description page
Abstract:
Here we present a protocol to propagate Homalodisca vitripennis cells and HoCV-1 in vitro. Medium was removed from HoCV-1 positive cultures and RNA extracted every 24 hr for 168 hr. Cell survivability was quantified by trypan blue staining. Whole virus particles were extracted post-infection. Extracted RNA was quantified by qRT-PCR.
Reading Level:
For undergraduate, graduate, and professional students
Subject Term:
Electronic Access:
https://www.jove.com/t/51953