TransFLP - A Method to Genetically Modify Vibrio cholerae Based on Natural Transformation and FLP-recombination
Title:
TransFLP - A Method to Genetically Modify Vibrio cholerae Based on Natural Transformation and FLP-recombination
Author:
Blokesch, Melanie
Personal Author:
Publication Information:
Cambridge, MA MyJoVE Corp 2016
Physical Description:
online resource (733 seconds)
Series:
Immunology and Infection
General Note:
Title from resource description page
Abstract:
A quick method to modify the genome of V. cholerae is described. These modifications include the deletion of single genes, gene clusters and genomic islands as well as the integration of short sequences (e.g. promoter elements or affinity-tag sequences). The method is based on the natural transformation and FLP-recombination.
Reading Level:
For undergraduate, graduate, and professional students
Subject Term:
Electronic Access:
https://www.jove.com/t/3761