Generation, Amplification, and Titration of Recombinant Respiratory Syncytial Viruses
Title:
Generation, Amplification, and Titration of Recombinant Respiratory Syncytial Viruses
Author:
Bouillier, Camille
Personal Author:
Publication Information:
Cambridge, MA MyJoVE Corp 2016
Physical Description:
online resource (708 seconds)
Series:
Immunology and Infection
General Note:
Title from resource description page
Abstract:
We describe a method for generating and amplifying genetically modified respiratory syncytial viruses (RSVs) and an optimized plaque assay for RSVs. We illustrate this protocol by creating two recombinant viruses that respectively allow quantification of RSV replication and live analysis of RSV inclusion bodies and inclusion bodies-associated granules dynamics.
Reading Level:
For undergraduate, graduate, and professional students
Subject Term:
Electronic Access:
https://www.jove.com/t/59218