Optimized Protocol for Efficient Transfection of Dendritic Cells without Cell Maturation
Title:
Optimized Protocol for Efficient Transfection of Dendritic Cells without Cell Maturation
Author:
Bowles, Robert
Personal Author:
Publication Information:
Cambridge, MA MyJoVE Corp 2016
Physical Description:
online resource (488 seconds)
Series:
Immunology and Infection
General Note:
Title from resource description page
Abstract:
We present our optimized high-throughput nucleofection protocol as an efficient way of transfecting primary human monocyte-derived dendritic cells with either plasmid DNA or siRNA without causing cell maturation. We further provide evidence for successful siRNA silencing of targeted gene RIG-I at both the mRNA and protein levels.
Reading Level:
For undergraduate, graduate, and professional students
Subject Term:
Electronic Access:
https://www.jove.com/t/2766