Preparation of Single-cell Suspensions for Cytofluorimetric Analysis from Different Mouse Skin Regions
Title:
Preparation of Single-cell Suspensions for Cytofluorimetric Analysis from Different Mouse Skin Regions
Author:
Broggi, Achille
Personal Author:
Publication Information:
Cambridge, MA MyJoVE Corp 2016
Physical Description:
online resource (358 seconds)
Series:
Immunology and Infection
General Note:
Title from resource description page
Abstract:
The skin is home to a complex immune cell network. We describe an efficient methodology for the digestion of mouse skin, from different parts of the animal's body, in order to obtain a single-cell suspension and analyze the different leukocyte populations resident in the skin by flow cytometry.
Reading Level:
For undergraduate, graduate, and professional students
Subject Term:
Electronic Access:
https://www.jove.com/t/52589