Culturing Microglia from the Neonatal and Adult Central Nervous System
Title:
Culturing Microglia from the Neonatal and Adult Central Nervous System
Author:
Bronstein, Robert
Personal Author:
Publication Information:
Cambridge, MA MyJoVE Corp 2016
Physical Description:
online resource (688 seconds)
Series:
Immunology and Infection
General Note:
Title from resource description page
Abstract:
We outline methods for the efficient and quick isolation/culture of viable microglia from the neonatal cerebral cortex and adult spinal cord. The dissection and plating of cortical microglia can be accomplished within 90 minutes, with the subsequent microglial harvest taking place ~ 10 days following the initial dissection.
Reading Level:
For undergraduate, graduate, and professional students
Subject Term:
Electronic Access:
https://www.jove.com/t/50647