Cover image for Peptide Scanning-assisted Identification of a Monoclonal Antibody-recognized Linear B-cell Epitope
Peptide Scanning-assisted Identification of a Monoclonal Antibody-recognized Linear B-cell Epitope
Title:
Peptide Scanning-assisted Identification of a Monoclonal Antibody-recognized Linear B-cell Epitope
Author:
Chen, Chien-Wen
Personal Author:
Publication Information:
Cambridge, MA MyJoVE Corp 2016
Physical Description:
online resource (489 seconds)
Series:
Immunology and Infection
General Note:
Title from resource description page
Abstract:
Here, the authors present a simple and efficient protocol to define a linear antigenic epitope using a purified monoclonal antibody and peptide scanning through dot-blot hybridization. The identified epitope can then be used in therapeutic and diagnostic applications.
Reading Level:
For undergraduate, graduate, and professional students
Electronic Access:
https://www.jove.com/t/55417
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