Measurement of Specific Mycobacterial Mistranslation Rates with Gain-of-function Reporter Systems
Title:
Measurement of Specific Mycobacterial Mistranslation Rates with Gain-of-function Reporter Systems
Author:
Chen, Yu-Xiang
Personal Author:
Publication Information:
Cambridge, MA MyJoVE Corp 2016
Physical Description:
online resource (378 seconds)
Series:
Immunology and Infection
General Note:
Title from resource description page
Abstract:
In this article, we present two complementary methods to measure specific rates of translational error and mistranslation in the model mycobacterium, Mycobacterium smegmatis, using gain-of-function reporter systems. The methods can be employed to measure accurate error rates in low throughput or relative error rates in a more high-throughput setting.
Reading Level:
For undergraduate, graduate, and professional students
Subject Term:
Electronic Access:
https://www.jove.com/t/59453