Identifying DNA Mutations in Purified Hematopoietic Stem/Progenitor Cells
Title:
Identifying DNA Mutations in Purified Hematopoietic Stem/Progenitor Cells
Author:
Cheng, Ziming
Personal Author:
Publication Information:
Cambridge, MA MyJoVE Corp 2016
Physical Description:
online resource (666 seconds)
Series:
Immunology and Infection
General Note:
Title from resource description page
Abstract:
Here we describe an in vivo mutagenesis assay for small numbers of purified hematopoietic cells using the LacI transgenic mouse model. The LacI gene can be isolated to determine the frequency, location, and type of DNA mutants spontaneously arisen or after exposure to genotoxins.
Reading Level:
For undergraduate, graduate, and professional students
Subject Term:
Electronic Access:
https://www.jove.com/t/50752