Contents -- Preface -- I. CLINICAL AND DIAGNOSTIC ASPECTS OF LEGIONNAIRES' DISEASE -- 1. Clinical Features of Legionnaires' Disease: A Selective Review -- 2. Treatment of Legionnaires' Disease -- 3. Diagnostics and Clinical Disease Treatment: Usefulness of Microbiological Diagnostic Methods for Detection of Legionella Infections -- 4. Hospital-and Community-Acquired Legionella Pneumonia: Two Faces of the Same Disease? -- 5. Risk Factors for Mortality by Legionnaires' Disease (1983-2005) -- 6. Trends Observed in Legionnaires' Disease in a Hospital in Catalonia, Spain, 1983-2005 -- 7. Community-Acquired Pneumonia in Human Immunodeficiency Virus-Infected Patients: Comparative Study of Streptococcus pneumoniae and Legionella pneumophila Serogroup 1 -- 8. Nosocomial Legionella Infection in the County of Copenhagen, 2000-2004 -- 9. A Question of Time: A Short Review of Data on the Incubation Period Between Exposure and Symptom Onset for Legionnaires' Disease -- 10. Severe Legionnaires' Disease Successfully Treated with Levofloxacin and Azithromycin -- 11. In Vitro Activities of Various Antibiotics against Legionella pneumophila -- 12. Detection of Legionella pneumophila DNA in Serum Samples from Patients with Legionnaires' Disease -- 13. Specific Detection of Legionella in Samples from Patients with Community-Acquired Pneumonia by PCR and a Colorimetric Detection System (Reverse Dot Blot) -- 14. Transcription-Mediated Amplification Assay for Detection of Legionella pneumophila in Samples from Patients with Community-Acquired Pneumonia -- 15. Detection of Legionella spp. and Legionella pneumophila- Specific DNA in Respiratory Secretions by PCR-Enzyme-Linked Immunosorbent Assay and Comparison with Conventional Methods -- 16. Serological versus Sequence-Based Methods for Legionella Identification.

17. Serologic Study of an Outbreak of Legionnaires' Disease: Variation of Sensitivity Associated with the Subgroup of Legionella pneumophila sg1 Antigen Used and Evidence of Concurrent Reactivity to Other Atypical Pneumonia Agents -- 18. Serotyping of Legionella pneumophila in Epidemiological Investigations: Limitations in the Era of Genotyping -- 19. Duopath Legionella: a New Immunochromatographic Test for Simultaneous Identification of Legionella pneumophila and Legionella Species -- 20. Antigenic Diversity of a 19-Kilodalton Peptidoglycan-Associated Lipoprotein among Legionella Species Determined by Reactivity Patterns to Monoclonal Antibodies -- 21. Evaluation of a New Rapid Immunochromatographic Test using Peptidoglycan-Associated Lipoprotein for Detection of Legionella Antigen in Urine Samples from Adults with Pneumonia -- 22. Rapid Identification of Legionella pneumophila, Legionella anisa, and Legionella taurinensis with Latex Agglutination Reagents -- 23. Clinical Presentation, Laboratory Diagnosis, and Treatment of Legionnaires' Disease -- II. EPIDEMIOLOGY AND STRAIN TYPING METHODS -- 24. Legionnaires' Disease in Europe 1995-2004: A Ten-Year Review -- 25. Typing of Legionella pneumophila and its Role in Elucidating the Epidemiology of Legionnaires' Disease -- 26. Effect of Legionella Testing Patterns on the Apparent Epidemiology of Legionnaires' Disease in Australia -- 27. 25 Years of Surveillance for Legionnaires' Disease in England and Wales:Why no Improvement? -- 28. Epidemiological Surveillance of Seropositive Legionellosis Cases in Korea During 1999-2002 -- 29. Prevalence of Legionnaires' Disease and Investigation on Risk Factors: Results of an Italian Multicentric Study -- 30. Seroprevalence of Antibodies to Legionella pneumophila in Northern Italy.

31. A Seroepidemiological Study of Legionella pneumophila Antibodies in Spanish Patients: A 13-Year Retrospective Study -- 32. Risk Differences of Legionnaires' Disease Associated with Travel in Spain, 1999 to 2004 -- 33. Persistence and Genotypic Stability of Legionella in a Potable-Water System in a Hotel over a 20-Month Period -- 34. 10 Years of Legionella Surveillance: Change of Legionella Subtype Preceded Epidemic of Nosocomial Legionnaires' Disease -- 35. Representative Survey of the Scope of Legionnaires' Disease and of Diagnostic Methods and Transmission Control Practices in Germany -- 36. Distribution of Legionella pneumophila Genotypes in Patients and Environmental Sources -- 37. Molecular Comparison of Isolates from a Recurring Outbreak of Legionnaires' Disease Spanning 22 Years -- 38. Sequence-Based Typing of Legionella pneumophila as an Aid in Investigation of Hospital-Acquired Legionnaires' Disease -- 39. Legionnaires' Disease Associated with Death after Near Drowning in Lake Water -- 40. Is use of Potting Mix Associated with Legionella longbeachae Infection? Results from a Case Control Study in South Australia -- 41. Epidemiological Typing of Legionella pneumophila in the Absence of Isolates -- 42. Online Identification of Legionella Species by DNA Sequence Analysis: the Macrophage Infectivity Potentiator Gene as an Example -- 43. Pulsed-Field Gel Electrophoresis Analysis and Sequence-Based Typing of Legionella pneumophila Serogroup 1 Isolates from Japan -- 44. Development of an Online Tool for European Working Group for Legionella Infections Sequence-Based Typing, Including Automatic Quality Assessment and Data Submission -- III. MICROBIOLOGY, PATHOGENESIS, IMMUNOLOGY, AND GENETICS -- 45. Identification of Translocated Substrates of the Legionella pneumophila Dot/Icm System without the use of Eukaryotic Host Cells.

46. Function of Legionella Effectors -- 47. The Legionella pneumophila Dot/Icm Type IV Secretion System -- 48. Subcellular Localization of the Dot/Icm Type IV Secretion Proteins -- 49. Defining the Translocation Pathway of the Legionella pneumophila Type IV Secretion System -- 50. Loss of a Patatin-Like Phospholipase A Causes Reduced Infectivity of Legionella Pneumophila in Amoeba and Machrophage Infection Models -- 51. Identification of a Cytotoxic Legionella pneumophila LpxB Paralogue in a Multicopy Suppressor Screen using Acanthamoeba castellanii as a Selective Host -- 52. Type II Protein Secretion and Twin-Arginine Translocation Promote the Pathogenesis of Legionella pneumophila -- 53. The Type II Protein Secretion System of Legionella pneumophila Is Important for Growth in Iron-Rich Media and Survival in Tap Water at Low Temperatures -- 54. Identification of Putative Substrates of the Legionella pneumophila Tat Secretion Pathway via Two-Dimensional Protein Gel Electrophoresis -- 55. Identification of Target Proteins of the Lss Secretion System of Legionella pneumophila Corby -- 56. Legionella pneumophila Mip: New Function for an Old Protein? -- 57. Phospholipases A of Legionella pneumophila: Virulence Factors by Diversity? -- 58. Identification and Characterization of Legionella pneumophila Phospholipases A -- 59. Characterization of GDSL-Hydrolases of the Lung Pathogen Legionella pneumophila -- 60. Genetic and Structural Examination of the Legiobactin Siderophore -- 61. Eukaryotic-Like Proteins of Legionella pneumophila as Potential Virulence Factors -- 62. Role of Legionella pneumophila-Specific Genes in Pathogenesis -- 63. The Hsp60 Chaperonin of Legionella pneumophila: an Intriguing Player in Infection of Host Cells -- 64. Lipopolysaccharide Architecture of Legionella pneumophila Grown in Broth and Host Cells.

65. Lag-1 Acetylation of Lipopolysaccharide -- 66. Immunochemical Analysis of Legionella pneumophila Outer Membrane Vesicles -- 67. Contribution of Legionella's Surface to the Pregnant Pause Virulence Strategy -- 68. New Insights into Pathogenesis of Legionella pneumophila Infection: from Bedside Findings to Animal Models -- 69. Induction of Apoptosis during Intracellular Replication of Legionella pneumophila in the Lungs of Mice -- 70. The Role of the Phagosomal Transporter (Pht) Family of Proteins in Legionella pneumophila Pathogenesis -- 71. A Role for Phosphoinositide Metabolism in Phagocytosis and Intracellular Replication of Legionella pneumophila -- 72. Interaction with the Ciliate Tetrahymena May Predispose Legionella pneumophila to Infect Human Cells -- 73. Genetics of Mouse Macrophage Resistance to Legionella pneumophila -- 74. Birc1e/Naip5 in Macrophage Function and Susceptibility to Infection with Legionella pneumophila -- 75. Locus on Chromosome 13 in Mice Involved in Clearance of Legionella pneumophila from the Lungs -- 76. Inflammatory Immune Response to Cytosolic Flagellin Protects Mice from Legionella pneumophila Infection -- 77. A Peptidoglycan-Associated Lipoprotein of Legionella pneumophila Activates Toll-Like Receptor 2 in Murine Macrophages -- 78. Legionella Infection of Bone Marrow Dendritic Cells Induces Modulation by Catechins -- 79. Gene Expression and Virulence in Legionella: the Flagellar Regulon -- 80. Identification of Legionella pneumophila Genes under Transcriptional Control of LpnR Regulatory Proteins -- 81. Modulation of rpoH Expression using an Antisense Strategy -- 82. Novel Use of Helicobacter pylori Nitroreductase (rdxA) as a Counterselectable Marker in Allelic Vector Exchange to Create Legionella pneumophila Philadelphia-1 Mutants.

83. Analysis of Gene Expression in Legionella during Axenic Growth and Infection.