Immunostaining of Whole-Mount Retinas with the CLARITY Tissue Clearing Method
Title:
Immunostaining of Whole-Mount Retinas with the CLARITY Tissue Clearing Method
Author:
Alessio, Elizabeth J.
Personal Author:
Publication Information:
Cambridge, MA MyJoVE Corp 2016
Physical Description:
online resource (541 seconds)
Series:
Neuroscience
General Note:
Title from resource description page
Abstract:
Here we present a protocol to adapt the CLARITY method of the brain tissues for whole-mount retinas to improve the quality of standard immunohistochemical staining and high-resolution imaging of retinal neurons and their subcellular structures.
Reading Level:
For undergraduate, graduate, and professional students
Subject Term:
Electronic Access:
https://www.jove.com/t/62178