Detection of Axonally Localized mRNAs in Brain Sections Using High-Resolution In Situ Hybridization
Title:
Detection of Axonally Localized mRNAs in Brain Sections Using High-Resolution In Situ Hybridization
Author:
Baleriola, Jimena
Personal Author:
Publication Information:
Cambridge, MA MyJoVE Corp 2016
Physical Description:
online resource (684 seconds)
Series:
Neuroscience
General Note:
Title from resource description page
Abstract:
RNA in situ hybridization (ISH) enables the visualization of RNAs in cells and tissues. Here we show how combination of RNAscope ISH with immunohistochemistry or histological dyes can be successfully used to detect mRNAs localized to axons in sections of mouse and human brains.
Reading Level:
For undergraduate, graduate, and professional students
Subject Term:
Electronic Access:
https://www.jove.com/t/52799