Dissection of Local Ca2+ Signals in Cultured Cells by Membrane-targeted Ca2+ Indicators
Title:
Dissection of Local Ca2+ Signals in Cultured Cells by Membrane-targeted Ca2+ Indicators
Author:
Bannai, Hiroko
Personal Author:
Publication Information:
Cambridge, MA MyJoVE Corp 2016
Physical Description:
online resource (693 seconds)
Series:
Neuroscience
General Note:
Title from resource description page
Abstract:
Here we present a protocol for Ca2+ imaging in neurons and glial cells, which enables the dissection of Ca2+ signals at subcellular resolution. This process is applicable to all cell types that allow the expression of genetically encoded Ca2+ indicators.
Reading Level:
For undergraduate, graduate, and professional students
Subject Term:
Electronic Access:
https://www.jove.com/t/59246