Correlative Light and Electron Microscopy to Study Microglial Interactions with β-Amyloid Plaques
Title:
Correlative Light and Electron Microscopy to Study Microglial Interactions with β-Amyloid Plaques
Author:
Bisht, Kanchan
Personal Author:
Publication Information:
Cambridge, MA MyJoVE Corp 2016
Physical Description:
online resource (652 seconds)
Series:
Neuroscience
General Note:
Title from resource description page
Abstract:
This article describes a protocol for visualizing amyloid Aβ plaques in Alzheimer's disease mouse models using methoxy-X04, which crosses the blood-brain barrier and selectively binds to β-pleated sheets found in dense core Aβ plaques. It allows pre-screening of plaque-containing tissue sections prior to immunostaining and processing for electron microscopy.
Reading Level:
For undergraduate, graduate, and professional students
Subject Term:
Electronic Access:
https://www.jove.com/t/54060