Laser Capture Microdissection of Neurons from Differentiated Human Neuroprogenitor Cells in Culture
Title:
Laser Capture Microdissection of Neurons from Differentiated Human Neuroprogenitor Cells in Culture
Author:
Bouchard, Ron
Personal Author:
Publication Information:
Cambridge, MA MyJoVE Corp 2016
Physical Description:
online resource (758 seconds)
Series:
Neuroscience
General Note:
Title from resource description page
Abstract:
Human neuroprogenitor cells (NPCs) were expanded under proliferating conditions. NPCs were differentiated into neuron-rich cultures in the presence of a combination of neurotrophins. Neuronal markers were detected by immunofluorescence staining. To isolate a pure population of neurons, NPCs were differentiated on PEN membrane slides and laser capture microdissection was performed.
Reading Level:
For undergraduate, graduate, and professional students
Subject Term:
Electronic Access:
https://www.jove.com/t/50487